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By the presence of 2 pairs of reciprocal recombinant molecules among the four spores of your tetrad (S11C and S11D Fig). Altogether, this revealed 37 masked COs (ranging from 0 to 16 per RTG strain) that did not bring about rLOH (S11E Fig), as well as the 77 COs leading to rLOH, which corresponds to the observed detection frequency of 77/(37+77) = 67.five , not substantially unique in the anticipa
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